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Using the model organism S. aureus, we have shown significantly increased detection rates using both FISH and molecular detection compared to cultivation techniques.
To evaluate the accuracy of the molecular detection compared to conventional culture, we considered only concordance with those samples with positive cultures for any of the organisms specified in the kit.
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Hence, it was the aim of this study to compare the conventional and molecular detection of P. aeruginosa from the sputum of adult patients attending the adult centre in Northern Ireland, as well as to estimate the lag time of conventional culture to detection, compared with molecular (PCR) detection.
The effect of screen detection compared with symptomatic diagnosis on survival was estimated after adjustment for grade, nodal status, Nottingham Prognostic Index (NPI) and the molecular markers.
Moreover, the detection of bacterial pathogen can mask an underlying viral cause; virus isolation may not always be successful and alternative methods of detection like IFAT can lack sensitivity and specificity compared to molecular detection methods.
For example, up to 73% of Plasmodium vivax infections were missed by microscopy, as compared with molecular detection, in hypoendemic areas of Brazil as the incidence of clinical cases declined.
The performance of the assay was compared with different molecular detection methods.
RT-PCR-based techniques demonstrated significantly lower sensitivity (adjusted P = 0.03) and no significant difference of specificity (adjusted P > 0.2) compared with other molecular detection methods.
Notably, RT-PCR-based protocols yielded significantly lower sensitivity compared with other molecular detection approaches and nested RT-PCR methods in particular demonstrated significantly lower specificity.
The present study was designed to use a PCR-RFLP protocol for the molecular detection of Anaplasma sp. and to compare its prevalence in blood samples from buffaloes (Bubalus bubalis) from 2 provinces of Pakistan and to determine the risk factors associated with the spread of Anaplasma infection.
Additionally, we sought to determine the specificity of contemporary biofilm detection methods by comparing them with molecular detection techniques in the same patients.
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