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On the other hand, molecular detection based techniques for bacteria identification are rapid, specific and sensitive.
We have designed a single-stranded DNAzyme aptamer sensor for homogeneous target molecular detection based on chemiluminescence resonance energy transfer (CRET).
However, development of molecular detection based on noninvasive methods is new and provides a definite and promising light for H. pylori therapy research.
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Molecular detection assays based on broadly reactive (degenerate) primers also tend to have differential and lower sensitivity for many flaviviruses.
In this paper, we describe the development of a molecular detection system, based on DNA array technology, for rapid and efficient detection of these vascular wilt pathogens.
RCA is a highly specific molecular detection mechanism based on DNA probe circularization.
Molecular detection methods based on the analysis of bacterial genomic DNA have become most frequently used to detect and differentiate tuber soft rot and blackleg pathogens in environmental samples.
In addition to immunocytochemistry, new molecular detection methods based on the amplification of a marker mRNA species by the polymerase chain reaction technique have been developed (Zippelius et al, JCO, 1997).
Viral molecular detection was based on TaqMan® assays previously described by Hernroth et al. (2002) for hAdV [ 16], Svraka et al. (2007) for NoV GI [ 17], and da Silva et al. (2007) for NoV GII [ 18].
The optimized reaction was subsequently used for the development of an assay for DNA detection based on a molecular-beacon probe.
Xiao et al. reported a system for DNA-target detection based on a molecular-beacon probe, which, after DNA-target hybridization to the MB loop, formed a peroxidase-mimicking DNAzyme that catalyzed ABTS oxidation [ 14].
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