The one-step nucleic acid amplification (OSNA) assay (Sysmex Corporation) is a rapid molecular detection procedure that analyses lymph-node metastases by detection and amplification of cytokeratin 19 (CK19) mRNA (Tsujimoto et al, 2007).
To analyse molecular detection of coliforms and shorten the time of PCR.
Molecular detection, biochemical identification, and cultural analyses of intestinal bacterial communities were performed as described [8], [14], [34].
Accurate microbiological diagnosis comprises molecular detection or quantitative buffy coat analyses, each of which enhances sensitivity.
Single cell 'omics' requires a technological platform with reliable and high throughput single cell analyses with single molecular detection and quantification.
The diagnosis of IE was based on the modified Duke's criteria [36] that include clinical data, blood cultures, immunohistochemical analyses of cardiac valves and molecular detection of organisms in blood and cardiac valves.
Culture positive chlamydial isolates were analysed using PCRII for subsequent molecular detection of C. suis.
Additional microbiological analyses, including fungal culture and the molecular detection of amoebas and herpes simplex virus (based on the 18S rDNA and DNA polymerase genes respectively), were negative.
In the meantime, additional microbiological analyses including fungal culture and the molecular detection of amoeba, bacteria, mycobacteria and herpes simplex virus (based on the 18S rDNA, 16S rDNA, rpoB and DNA polymerase genes, respectively) were negative.
In the meantime, additional microbiological analyses including fungal culture and the molecular detection of amoeba, mycobacteria and herpes simplex virus (based on the 18S rDNA, rpoB and DNA polymerase genes, respectively) were negative.
