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The tree file containing the maximum credibility clade tree with branch length (node age) generated by the molecular dating procedure, and trait files containing the discrete state for each genus were used for the analyses.
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We note that other avenues for molecular dating are being developed because the standard dating procedure results in overlaying two prior distributions for a calibration node: one from the tree prior and one from the calibration prior [ 19, 21, 102].
More details regarding this dating procedure, including its strengths and weaknesses with respect to other relaxed molecular clock methods (recently reviewed in [ 121]) can be found in Bininda-Emonds et al. [ 117].
The rhombus and star signs denote calibration points for molecular dating.
This makes very difficult to date the formation and the diversification of the CD complex by molecular dating methods.
A third issue in molecular dating involves the general modelling of among-lineage rate heterogeneity.
Biparental mitochondrial inheritance followed by recombination can complicate phylogenetic reconstruction and molecular dating [3], [4].
ESWW carried out molecular dating.
Molecular dating was done in two ways.
Eight fossil-based calibrations were utilized for molecular dating analyses.
Molecular dating of genes was accomplished using BEAST [ 57].
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