We conducted phylogenetic analyses using: (i) morphological characters only (80 species × 220 characters), (ii) molecular characters only (76 species × 6032 characters), and (iii) combined morphological and molecular data (80 species × 6252 characters).
Based on phenotypic and molecular data two species, minula and althaea, have been recognized as species distinct from curruca.
With respect to the molecular data, the species hypotheses put forth by Bond and Platnick [28] are also in conflict.
With respect to the utility of estimating N e from molecular data for species of conservation concern, numerous, highly polymorphic markers are also becoming more prevalent and new programs are able to capitalize on these advancements and make single point estimators more useful.
Despite the recent advances in generating molecular data, reconstructing species-level phylogenies for non-models groups remains a challenge.
In order to compare our molecular data with Plasmodium species previously found in lemurs, we included a species isolated from E. m. macaco identified at the Muséum National d'Histoire Naturelle, Paris, France [ 23].
These molecular data included 13 species representing all genera of old endemic Australian rodents, all 7 species of new endemics, and all 3 introduced species (Rattus and Mus).
The molecular data supported more species, some of them lacking morphological support, and the authors announced that decisions on species boundaries will be formalized in a forthcoming taxonomic monograph.
Clopton [23] recently evaluated the phylogeny of septate eugregarines using molecular data of 27 species isolated from several different insect hosts.
For this reason this study, like that of Astrin et al. [35], provides a generally straightforward case wherein species appear to be morphologically unambiguous and as such provide a framework that can be used to assess the efficacy of molecular data to recover species defined on the basis of genitalic differences.
