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For ancestral character state reconstructions, twelve diagnostic characters were analyzed on the posterior set of trees derived from the combined molecular data analysis (Fig. 2).
WR conceived of the study, participated in its design, and contributed to histological and molecular data analysis and to the manuscript.
TB carried out the establishment of all molecular methods and the experiments, performed the sequence alignments and all other genetic and molecular data analysis, captured the macroscopic, histological and SEM images and drafted the manuscript.
GB contributed to morphological data coding, analysis, and interpretation, SM and PB commented on molecular data analysis and interpretation, PB and NB contributed to earlier drafts of the manuscript with respect to data interpretation.
We are grateful to Dr. Richard G. Hodel (University of Florida) for assistance with the molecular data analysis, and Prof. Richard J. Abbott (University of St Andrews), Dr. Kenneth M. Olsen (Washington University in St . Louis, and three anonymous reviewers for their insightful comments and suggestions to improve the manuscript.
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Ancestral state reconstructions showed that the common ancestor of this clade was either unequivocally freshwater (morphological data, parsimony analysis) or likely freshwater (molecular data, likelihood analysis).
JG participated in the planning of the study, acquisition of samples, culture and isolation of mycobacteria and molecular analysis of isolates; BBA participated in planning of the study, training JG, supervision of the molecular assays, data analysis and drafting of the manuscript.
JN conceived the study, collected samples and molecular data, performed analysis and drafted the manuscript.
OMM and MMM were involved in data collection (molecular analysis), data analysis and interpretation.
MMM was involved in data collection (sample preparing, molecular analysis), data analysis and interpretation.
DEG supervised or carried out the molecular studies, data analysis, and drafted the manuscript.
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