PFPBO has a unique molecular structure containing a boron atom center with electron deficiency and an oxalate group.
The allosteric response of a β-galactosidase molecular sensor containing a B-cell epitope from HIV has been finely dissected upon binding of an effector monoclonal antibody, within a wide range of standard concentrations of both enzyme and substrate.
In order to enhance the stability and detection efficiency of the probe in biofluids, we design a shared-stem molecular beacon containing a 27-mer loop and a 4-mer stem with DNA/LNA alternating bases.
We found that the enzyme substrate recognition step is mainly controlled by apolar interactions and that a "good" substrate has a molecular structure containing a long aliphatic chain and a second positive charge at a distance greater than 12 Ǻ from the reactive amino group.
A 31-base long molecular beacon, containing a 15-base recognition sequence specific for the mutant target, was linked via a thiol modified poly thymine linker (10 bases long) to a 13 nm gold nano-particle and was exposed to mutant and wild type targets, and a clear differentiation was achieved at target concentrations as low as 1 nM.
In addition, to explain the strain mutation phenomenon, it has been recently proposed that a single strain may consist of an ensemble of molecular species containing a dominant PrPres type [21].
The N. vectensis and A. digitifera mitochondrial genome comprises molecular DNA containing a group I intron in the ND5 gene.
Of the two possible amplified fragments, only one was obtained of the expected molecular weight, containing a portion of 5'- LTR, indicating the occurrence of a Copia element in that side of the locus.
cDNA cassettes encoding either amino acids 3770 4007 of RyR1 (CLR-1) or amino acids 3620 3859 of RyR3 (CLR-3) were cloned into the pCold-II expression vector (Takara™) in-frame downstream of a molecular tag containing a streptavidin-binding peptide and a His6 tag.
As an alternative approach to increase the hapten density within the drugs of abuse vaccine arena, we envisaged the use of a small molecular scaffold containing a core that would allow diverging three-dimensional projection of the hapten copies to promote spatial separation.
Design of the system: The target design for the mechanically bonded host features the incorporation of the osmium II) tris bipyridine) reporter group into the macrocyclic component of a rotaxane molecular framework containing a convergent hydrogen-bond-donor anion-binding interlocked cavity.
