Sentence examples for molecular constructs were from inspiring English sources

The molecular constructs were cloned in Escherichia coli strain Top 10 and confirmed by molecular methods and were transfected by electroporation into Leishmania major.

In this study, as part of a long-term goal to examine the protein interactions of Cas by X-ray crystallography and nuclear magnetic resonance spectroscopy, molecular constructs were designed to express two adjacent domains, the serine-rich domain and the Src-binding domain, that each participate in intermolecular contacts dependent on protein phosphorylation.

Various molecular constructs were derived from psiCHECK™-2 (Promega) by cloning behind the renilla luciferase ORF sequences from fgf7 3'-UTR mRNA.

25 Molecular constructs were confirmed by sequencing.

Note that all molecular constructs were based on the 695 amino acid APP isoform.

All the molecular constructs were prepared by using standard molecular biological techniques [ 29].

E. coli DH5α (TOYOBO, Osaka, Japan), used as a host cell for molecular constructs, was cultured overnight in LB medium (2.0% tryptone, 1.0% yeast extract, 2.0% NaCl) at 37 °C.

These free linear molecules, which each contained 10 distinct segments and a vector backbone, were circularized via an intra-molecular ligation; the circular constructs were then used to transform E. coli.

Standard molecular techniques used to make constructs were as described in Sambrook and Russell [ 48].

While some of the molecular constructs that are used for these experiments require a bit of patience to understand, Maher et al. (2013) highlights many important concepts that are worthwhile for introductory genetics students.

The nomenclature for the molecular constructs employed is shown in Table 1.