All constructs described are available upon request.
All constructs described were subcloned into pcDNA3.1.
The YFP STIM1 NN) mutant was constructed using standard molecular biology procedures and based on the construct described previously [ 22].
Hence, overall system optimisation is difficult, even though it is urgently required in order to describe and understand the behaviour of new molecular constructs.
25 Molecular constructs were confirmed by sequencing.
PAS, RUR, CR and GDS made the molecular constructs.
All the molecular constructs were prepared by using standard molecular biological techniques [ 29].
AAC and JAJD generated the molecular constructs and performed the molecular and transient expression assays.
In light of this widespreadness of the involvement of multisite phosphorylation in regulating protein degradation, it appears plausible that the molecular mechanism described by our model might represent a common design principle utilized by nature for constructing protein degradation switches.
Thermal denaturation profiles of both molecular beacons were constructed as described in Materials and Methods and are seen in Figure 3.
