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For injections of molecular constructs, capped synthetic mRNAs encoding a dominant-negative serotonin receptor 3A and mRNAs encoding β-galactosidase, a lineage tracer, were dissolved in water.
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25 Molecular constructs were confirmed by sequencing.
PAS, RUR, CR and GDS made the molecular constructs.
All the molecular constructs were prepared by using standard molecular biological techniques [ 29].
AAC and JAJD generated the molecular constructs and performed the molecular and transient expression assays.
An increase in the low temperature (polyisoprene-type) Tg value was observed for the lower molecular weight zwitterion capped polymers.
For Xenopus laevis oocyte experiments, NR1-1a aNR2BR2B constructs and capped cRNAs were generated as described previously.
Three genotypes were assayed for association of the LacO array with NR events: Cap-H2 overexpression (LacO, GFP-LacI; HS > GAL4, UAS > Cap-H2) crossed to flies with the tether construct, Cap-H2 overexpression (LacO, GFP-LacI; HS > GAL4, UAS > Cap-H2) crossed to flies without the tether construct, and GAL4 control (LacO, GFP-LacI; HS > GAL4) crossed to flies with the tether construct.
The resultant constructs, pHis-Cap, pGST-Cap and pTrx-His-Cap were then individually transformed into three distinct E. coli strains in order to address the effect of the fusion tags on the protein expression levels of the cap gene.
RS shows promise for application in the diagnosis and grading of CaP in clinical practise as well as providing molecular information on CaP samples in a research setting.
The difunctional polyether used had a molecular weight of 2300 g/mol end capped with 20 wt% ethylene oxide.
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