Together, these factors can confound attempts to infer network architectures and gain molecular insights into commitment and subsequent lineage specification from averaged gene expression profiles.
This approach allows timed sampling after instigation of a discrete lineage trigger, and it may provide a more homogeneous molecular entry into commitment and lineage development.
Although the molecular basis for floral commitment remains unclear, recent studies suggest that the persistent activity of FLOWERING LOCUS T (FT) at inflorescences is required for floral commitment in Arabidopsis thaliana.
However, the molecular mechanisms controlling ESC commitment toward specific lineages need to be specified.
To achieve this goal, molecular mechanisms controlling MSC commitment to ADs versus osteoblasts need to be identified.
Therefore, there is a crucial need for a better understanding of molecular mechanisms governing ESC commitment into specific lineages.
The transcriptional heterogeneity observed within individual populations may highlight the capture of multiple contemporaneous molecular programs underlying lineage commitment under self-renewal conditions.
Recent efforts in deciphering this highly complex code in cellular differentiation are beginning to shed light on the molecular mechanisms of lineage commitment and differentiation potential during development [ 16- 20].
Combined, these data suggest that activation of PU.1 expression during developmental haematopoiesis plays a role in driving a haematopoietic rather than endothelial transcriptional programme, and that activation of PU.1 expression in haemogenic endothelium might be an important molecular decision in haematopoietic commitment.
Thus, much effort has been focused on finding molecular targets that drive oligodendrocytes commitment and development.
