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In a second approach using molecular combing technologies, genomic DNA was obtained from a control lymphoblastoid cell line.
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Combing was performed using the Molecular Combing Apparatus™ (Institut Pasteur, Paris, France and Genomic Vision) on silanized surfaces[ 92].
Microfibrils derived from diabetic tissues were fragmented, morphologically disrupted and weakened (as assessed following molecular combing).
The hydrophobic surfaces for molecular combing are generally functionalized using silane chemistry.
Hence in this study, we employed molecular combing to apply a capillary tensile force to partially adsorbed microfibrils (Fig. 7a).
DNA molecular combing coupled with FISH was performed as described in Suram et al (2012) with minor modifications.
The DNA fibres were mechanically stretched onto saline-coated coverslips using a Molecular Combing System (Genomic Vision).
The current diagnostic techniques for FSHD1 include pulsed-field gel electrophoresis (PFGE) and molecular combing [ 78, 79].
Single-molecule DNA fibres were prepared by molecular combing (34) according the manufacturer's instructions (Genomic Vision).
RCA products are stretched by molecular combing, and 10 nm AuNPs are aligned along the DNA strand by hybridization of a complementary sequence attached to the particles.
Fluorescence in situ hybridization (FISH) on interphasic nuclei and molecular combing techniques allowed to approximate the size and the organization of the 8q24.3 ZNF cluster.
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