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However, Nomura (2008) showed that considerable improvements in performance can be obtained even by taking an unweighted harmonic mean of from the heterozygote excess and molecular coancestry methods.
Figure 4 represents the joint distribution of estimates from the heterozygote-excess and molecular coancestry methods applied to the inbred populations under polygyny with Nm = 5 and Nf = 20 parents and L = 15 high-polymorphic marker loci.
Left and middle panels in Fig. 1 (A: monogamy and B: polygyny) illustrate the 10th, 50th and 90th percentiles, and a harmonic mean of 5000 replicated estimates of the effective number of breeders (Neb) from the heterozygote-excess and molecular coancestry methods applied to the noninbred population with L = 5 20 high-polymorphic marker loci.
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The molecular coancestry method could significantly contribute to the detection of small Neb of such populations.
Thus, the molecular coancestry method gives an essentially unbiased estimate of Neb for the noninbred population (Fig. 1).
In contrast, the molecular coancestry method gave an estimate with a practically acceptable confidence interval when L = 30 marker loci were available.
Although the molecular coancestry method will be less useful for a point estimate of Neb in inbred populations, it will be useful for detecting a small Neb.
In all the cases computed, the 90th percentile in the molecular coancestry method was much smaller than in the heterozygote-excess method.
However, the process of selecting putative nonsibs in the molecular coancestry method causes a problem when applied to the inbred population.
Although further evaluations including other published methods for sib-ship reconstruction should be carried out under a wide range of scenario, the results in Table 4 suggest the potential for improving the molecular coancestry method.
Although the 50th percentile and harmonic mean show that the heterozygote-excess method gives an essentially unbiased estimate of Neb, the estimate from the molecular coancestry method tends to be biased downward.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com