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These developments have been boosted by the molecular cloning, development and implementation of a jelly-fish-derived green fluorescent protein.
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These genes are the most suitable candidates for molecular cloning and development of new functional gene-target markers to facilitate marker assisted breeding.
Yet, in certain cases (such as attempts to generate attenuated strains of viruses via molecular cloning for vaccine development), it has become important to evaluate the effects of sequence changes on the viability of the virus.
With the development of molecular cloning for the identification of cellular RNA fragments and deep sequencing, a number of classes of small RNAs have been identified recently, including a novel class of small RNAs in eukaryotes.
Methods for the diagnosis of infection with human caliciviruses (HuCVs) have undergone two major stages of development: before and after molecular cloning of Norwalk virus (NV).
We identified sequences of nine Hox genes and the ParaHox gene caudal through a combination of transcriptome analysis and molecular cloning, and determined their expression pattern during development using in situ hybridization in whole-mounted larvae.
Additionally, advances in molecular cloning and expression of peptides in display technologies could allow for the rapid development of NCR products as soon as resistance occurs in insect populations in the field.
Given the importance of Otolin in lower organisms, the molecular cloning and biochemical characterization of the mammalian Otolin protein may lead to a better understanding of otoconial development and vestibular dysfunction.
Molecular cloning of functional immunoglobulin genes from single plasma cells is one of the most promising technologies for the rapid development of monoclonal antibody drugs.
YZ performed molecular cloning and protein expression.
Overview of molecular cloning of membrane protein markers.
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