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Phylogenetic reconstruction at each gene region deviated significantly from the expectations of a molecular clock (evaluated by standard likelihood ratio test).
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For divergence time estimation, the molecular clock was evaluated using a two-cluster test in the LINTREE program [ 45].
Furthermore, we used a molecular clock approach to evaluate the evolutionary time frame of diversification in phyllostomid bats.
Sanders, K. L. & Lee, M. S. Y. Evaluating molecular clock calibrations using Bayesian analyses with soft and hard bounds.
However, its potential role in synchronizing the molecular clock that underlies somite formation was not evaluated in murine embryos.
Use of this molecular clock model provides a measure of rate heterogeneity among lineages or how well data conform or deviate from a strict molecular clock [ 52].
The implementation of a relaxed molecular clock enabled us to estimate mitochondrial substitution rates and to evaluate the effect of life history traits on it.
Maximum likelihood trees generated for the full dataset, as well as trees based exclusively on the third codon position, or on the first and second codon positions were also evaluated under the null model (no clock) and alternative models (molecular clock enforced).
Likelihood ratio tests [26] evaluate whether a phylogenetic tree estimated without enforcing a molecular clock is significantly more likely than a tree estimated when a clock is enforced [e.g. 6].
In order to compare rates of Xist evolution in the above lineages, we used a likelihood ratio test (LRT) to evaluate the hypothesis that these genes were evolving according to a molecular clock [69].
Prior, the relaxed uncorrelated lognormal molecular clock model [ 106] was implemented to assess the clock-like nature of the data.
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