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Based on the silent site mutation rate for D. pulex of 6 × 10− mutations per base per year (Tucker et al. 2013) and the molecular clock estimation method described in the Materials and Methods section, we estimated the approximate age of the MRCA of all sampled D. pulex clones to be 3.4 × 10 years.
Molecular clock estimation suggested the Japanese angelshark lineage diverged from the rest of the Asian angelsharks some 100 million years ago during the Cretaceous.
Based on molecular clock estimation, Trigonognathus is thought to have originated around 41 million years ago during the Middle Eocene, as part of a larger evolutionary radiation of etmopterid genera.
Molecular clock estimation placed the speciation of the tasselled wobbegong at 11 6 Ma, coinciding with a period of significant geological rearrangement and the formation of coral reef habitats in the region.
These findings indicate inconsistencies in molecular clock estimation, which we further explored.
Conventional molecular clock estimation led to an unrealistic early dating of the start of the domestication.
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The paleobiographic scenario envisaged is corroborated also by molecular clock estimations and correlations with Late Neogene environmental changes in the Mediterranean region which ended with the Messinian salinity crisis.
Nonetheless, molecular clock estimations provided variable results on the timing of ariine diversification (~105-41 mya).
If that is the case, a precise assignment of the oldest known specimens to particular ariid subclades might provide more accurate molecular clock estimations in support of the vicariant scenario.
Molecular clock estimations suggest that this group diversified in a fairly short time period during or after the Messinian salinity crisis at the end of the Miocene (4 4.5 mya).
The uncertainties and large stochastic errors associated with the molecular clock estimations preclude the correlation of the tree node ages with the datings of particular geological and climate transitional episodes.
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