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Additional file 2: Linearized molecular clock dated tree (approach 1).
The linearized molecular clock dated maximum-clade-credibility tree shows the relationships of selected mt lineages (concatenated 16S, 12S and COI sequences ).
The calculations of molecular clock dated linearized BI trees were performed in BEAST v.1.7.5 [ 25] with the concatenated mt sequences of COI, 12S and 16S.
The molecular clock dated trees were calculated in BEAST v1.7.5 [ 25] with the three mt markers (COI, 16S and 12S), using Sphyradium doliolum, Orculella bulgarica (Hesse, 1915) and Orculella aragonica (Westerlund, 1897) as outgroups.
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Molecular clock dating indicates it reached Haiti roughly in 1966.
Molecular clock dating shows that M reached humans somewhere near 1921.
The oldest fossil "proto-frog" appeared in the early Triassic of Madagascar, but molecular clock dating suggests their origins may extend further back to the Permian, 265 million years ago.
For molecular phylogenetics and molecular clock dating, total DNA was extracted from wild-caught and lab-reared Deltophora adults and larvae using standard methods.
DNA sequences placed the moths and plants phylogenetically and confirmed that larvae were those of the pollinating moths; molecular clock dating suggests that the moth clade is younger than the plant clade.
However, as with molecular clock dating of viruses, associated confidence limits should not be overlooked.
This number is used as a calibration point for molecular clock dating using BEAST.
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