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Molecular clock calibrations based on biogeographic landmarks indicate that the lineage leading to A. persimilis diverged from the common ancestor of all Artemia species between 80 and 90 MYA at the time of separation of Africa from South America, whereas parthenogenesis first appeared at least 3 MYA.
Based on the biogeography and molecular clock calibrations, this subspecies may have been separated from the remainder of the distribution since the Miocene, molecular clock calibrations estimating 13.9 million years of separation.
Molecular clock calibrations indicate that pellonuline herring reached Madagascar 48 MYA (95% reliability interval: 34.0 66.2).
Existing molecular clock calibrations for notothenioids are based on few mitochondrial markers in combination with a single putative, but debated, eleginopid fossil [22], biogeographic patterns [23], or the presumed date of the perciform diversification [24].
Molecular clock calibrations and confidence intervals were based on paleontological data from the oldest (K-T boundary) leaf beetle fossil, ancient feeding traces ascribed to hispoid Cassidinae, and the vicariant split of Nearctic and Palearctic members of the Timarchini.
Based on molecular clock calibrations and fossil evidence (Text S7) showing that Labrini were present in the Mediterranean 14.0 Ma, it has been suggested that the ancestor of Labrini migrated from the Indopacific into the Mediterranean prior to the closure of this seaway, 20.5−19.5 Ma [27].
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Molecular clock calibration followed the protocols outlined in Rutschmann [65].
Molecular clock calibration is always problematic, but this >10-fold difference offers dramatic contrast between a recent and ancient New World introduction.
To enable comparison of our molecular clock calibration based on intraspecific variation with other estimates, the time represented by the scale bar in figure 3 was determined using other calibration methods.
Taken together the population genetic structure, source attribution of isolates and molecular clock calibration for clade divergence are consistent with an evolutionary scenario where a lineage within the C. coli has diverged into an agricultural niche.
Within the Coleoptera, the family Carabidae has been the focus of several molecular clock calibration attempts.
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