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However, a gap has recently been suggested to exist in the evolutionary rates of mtDNA gene sequences between interspecific divergence and intraspecific polymorphism and, therefore, the assumption that a single molecular clock applies throughout all lineages over time may not be correct (e.g. [ 1– 4]).
We show ILS level (given in terms of average bipartition distance between true gene trees and true species trees, expressed as a percentage), number of sites per gene, number of genes, whether a strict molecular clock applies, and number of replicate species trees.
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To investigate whether a global molecular clock applied to our data, we conducted under PAUP*4.0b10 [ 66] a likelihood ratio test between log-likelihoods of clock-constrained and non-constrained trees.
Based on CR mtDNA sequence analyses and calibration of molecular clock applied to the nucleotide divergence of these sequences between the major grayling clades with a CR mutation rate of 1% per million years (MY), Froufe et al. [ 4] have dated the colonization of Europe to the Pliocene-Pleistocene boundary around 4.6 to 1.6 million years ago (MYA), far before the onset of Pleistocene ice age.
An uncorrelated lognormal relaxed molecular clock was applied.
An uncorrelated lognormal relaxed molecular clock was applied to each partition.
A relaxed molecular clock is applied to the phylogenies and key divergence dates estimated by a Bayesian approach.
Both a strict and a relaxed molecular clock were applied; the results were similar and a strict clock was used for the final runs.
If a molecular clock is applied to the sequence divergence, we estimate an initial divergence from a common ancestor approximately 2 mya.
We carried out the diversification analyses on a random sample of 100 trees obtained from a relaxed molecular clock analysis, applying a pure-birth process after model selection.
The analyses with Beast differed from those with MrBayes in that, with Beast, a molecular clock was applied to the tree and specified calibration point (date) priors were used in addition to substitution model parameter priors.
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