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We ran a series of molecular clock analyses in r8s v.1.81 (ref. 70).
Zimmer, A. et al. Dating the early evolution of plants: detection and molecular clock analyses of orthologs.
The team also conducted molecular clock analyses to identify when the three P. robustus forms diverged and found consistent estimates for the most recent common ancestor.
It should be noted, that divergence times estimated from molecular clock analyses largely depend on the values used to define the prior distribution on the substitution rate.
Comparing multiple molecular clock analyses, we find that the maximum marginal probability for the divergence time of bacterial and eukaryal sterol biosynthesis genes is around 2.31 Gyr ago, concurrent with the most recent geochemical evidence for the Great Oxidation Event7.
Relaxed molecular clock analyses indicated species radiations of this clade concentrated at approximately 1.9 7.6 MYA.
Further support for our hypothesis comes from divergence times inferred via molecular clock analyses.
Molecular clock analyses were performed to infer the divergence time for the Cathorops sp./C.
This more ancient timeframe has been corroborated from molecular clock analyses of rbcL data [29].
Additionally, divergence times were estimated via molecular clock analyses and morphological variation quantified using morphometric and meristic approaches.
Two molecular clock analyses were then performed on the alignment of 107 brown bears, using the model settings described above.
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