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Cultivar differentiation was performed using molecular characterization with ISSR and SSR markers.
Molecular characterization with high accuracy, sensitivity and economics is required for population screening and genetic counseling.
Seven of these isolates underwent additional molecular characterization with PCR and multilocus sequence typing by using methods previously described (1, 3 ).
All the isolates confirmed as N. meningitidis were subject to molecular characterization with the exception of 842 serogroup X isolates collected in the district of Kaya.
The isolate (Mycobacterium bovis) correlates by molecular characterization with other isolates from wild ungulates in the park, strongly suggesting an epidemiologic link.
Rotavirus-reactive samples were stored at −70°C until their molecular characterization with reverse transcription-polymerase chain reaction (RT-multiplex PCR) [ 24, 25].
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These ciPSC lines were shown to express OCT4/ALP and expressed FLK-1, AFP and TUBB3 after rapid differentiation; however, the ciPSC lines still require the proper molecular characterization in concordance with accepted standards within the field [56], [57].
Our large study on molecular characterization of MUP with nodal metastases showed that MUPs had molecular features similar to sporadic non-chronic-sun-damaged melanomas.
AFLP fingerprinting is useful for the molecular characterization of microorganisms with relatively large genomes, including various fungal species (18, 19, 21 – 29, 29, 30 ).
Molecular characterization was performed with a crude biosurfactant mixture extract, which was dialyzed against demineralized water at 4°C in a Spectrapor membrane tube (molecular weight cut off 6000 8000, Spectrum Medical Industries Inc., CA, USA), and then freeze-dried.
In this regard, our work provides new insights into the molecular characterization of children with NS that could potentially be exploited for more advanced screening and monitoring of children with RASopathies and MPDs.
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