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Bacterial isolates underwent molecular characterization using multilocus sequence typing, staphylococcal cassette chromosome mec (SCCmec) typing, and PCR for the presence of the genes encoding Panton-Valentine Leukocidin (PVL).
Upon further molecular characterization using multiplex PCR, 68% (21/31) of the colonies showed a positive signal for the genus Mycobacterium, of which two were confirmed Mycobacterium bovis (M. bovis) by RD4 deletion typing.
Then, molecular characterization using 16S rRNA sequencing analysis was performed.
RNA was eluted in a final volume of 60 μL for downstream molecular characterization using qRT-PCR.
Based on molecular characterization using VP1 gene sequences, EV71 was classified into genotypes A, B, and C (Fig. 1) (79).
Twenty-six of the 42 influenza A positive samples were randomly selected for molecular characterization using direct nucleotide sequencing of the HA gene.
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The fusant obtained on the basis of colony morphology subjected to molecular characterization by using RNA profiling study.
Surveillance in vectors could also be considered for virus detection and isolation which would allow further characterization using molecular methods.
We carried out an extensive examination of all Arabidopsis FYVE domains, including their identification, classification, molecular modeling and biophysical characterization using computational approaches.
A genome-wide germplasm characterization using molecular markers can offer reliable tools for adjusting the quality and representativeness of such core samples.
To date and including our case, only six cases of central system nervous infection by N. otitidiscaviarum have been reported in the literature [ 5, 7- 10], without any taxonomic characterization using molecular techniques such as 16S rRNA gene sequencing.
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