Molecular characterisation was performed using isoelectric focusing and polymerase chain reaction (PCR) with sequencing, whilst strain relatedness was determined by pulsed-field gel electrophoresis (PFGE) using XbaI.
Molecular characterisation was done using the following techniques: isoelectric focusing; polymerase chain reaction (PCR) and sequencing of blaESBL; PCR for plasmid-mediated quinolone resistance determinants; identification of ST131; phylogenetic grouping; and replicon typing.
After this initial characterisation, the strains were further characterised using molecular methods.
The bacterial isolates ST34, identified as B. amyloliquefaciens (collection number SARCC 696 at the South African Rhizobium Culture Collection) and ST5, identified as P. aeruginosa (collection number SARCC 697 at the South African Rhizobium Culture Collection), using molecular characterisation (Ndlovu et al. 2016), were utilised in the current study.
The bacterial isolates ST34, identified as B. amyloliquefaciens (collection number SARCC 696 at the South African Rhizobium Culture Collection) and ST5, identified as P. aeruginosa (collection number SARCC 697 at the South African Rhizobium Culture Collection), using molecular characterisation (Ndlovu et al. 2016), were utilised in the current study for biosurfactant production.
Molecular characterisation of microorganisms can be used to provide evidence of epidemiological relationships between strains and is an important tool in the investigation of the spread of infectious diseases [ 4].
Transgenic seeds of T0 plants numbered 1, 2 and 3 were collected and used for molecular characterisation, an in vitro inhibitory assay, and the production of T1 plants from plants 2 and 3.
The objectives of this study were to conduct an initial molecular characterisation of M. bovis in Mali using spoligotyping and to identify potential exchange of strains with other regions.
For the molecular characterisation of RV strains we have used PCR/nested amplification and direct sequencing of a 513-nucleotide region of the E1 gene.
A number of typing tools have been used for the molecular characterisation of proteolytic C. botulinum.
