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As the dominant marker could not differentiate the homozygous and heterozygous status of the alleles, which would limit its application in molecular breeding program.
While Saltol is a promising QTL for seedling stage salinity tolerance, its specific role in controlling Na+/K+ homeostasis and the presence of different Pokkali alleles in the Saltol region calls for a careful strategy for transferring tolerant alleles into new varieties as part of an integrated molecular breeding program.
As the EMS mutants are nontransgenic, subsequent generations can be grown under field conditions, without restrictions, for phenotypic analysis and advantageous alleles can be immediately incorporated into a fast-paced molecular breeding program using the characterized induced mutations as markers for selection.
For molecular breeding program of sugarbeet, its recently available genome assembly (569 Mb) of KWS2320 genotype (3) needs in silico approach for bulk marker discovery.
If STR markers from our database are used for mapping of these genes or markers of flanking regions of these specific genes are selected, then they can directly be used in molecular breeding program for introgression of these genes/traits.
Fourthly, the converted markers were tested on a large number of individual plants of a segregating population to confirm the genetic linkage between the markers and the gene of interest before the markers were confidently implemented into a molecular breeding program.
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These data will facilitate both the positional cloning of the R gene and molecular breeding programs of rice blast resistance.
The results were used to develop a regression equation model that can be used for rice molecular breeding programs.
It is indeed that JHN has been widely used as an elite resistant donor in conventional and molecular breeding programs against rice blast disease in Thailand.
This provides a basis for future research on the elucidation of the genetic mechanisms underlying SD in rice, and will be useful in molecular breeding programs.
Thus, it is important to explore the potentially favorable alleles of Gn1a to improve rice yield when designing strategies in molecular breeding programs.
Related(13)
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