The molecular beacon based method is likely to be much better than the antibody-based method in that the availability of target molecules (mRNAs) is quite large and the cost of synthesizing molecular beacons is low compared with that of generating antibodies (even if multiple cell surface protein markers are available).
In this work, we develop a fluorescent molecular beacon based on the DNA-templated silver nanoclusters (DNA-Ag NCs).
In this paper, a molecular beacon based on silver ions-mediated cytosine-Ag+-cytosine base pairs was used to detect DNA.
In respect of molecular beacon based aptasensor, FRET between Trp3a-1 labeled with FAM and CS-Trp3a-1 labeled with BHQ-1 black BHQ-1 blacker-1) whole is partially complementary with the aptamer was used to detect L-Trp.
Three duplex molecular beacon based real-time Nucleic Acid Sequence Based Amplification (NASBA) assays have been designed and experimentally validated targeting RNA transcripts for the detection and identification of Haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae respectively.
In summary a new LNA molecular beacon based real time RT-PCR assay was used successfully to measure quantitatively the total level of HCV RNA in both experimental and clinical specimens.
In brief, the molecular beacon based PCR assay described here meets the essential requirements for use in resource limited settings.
As a positive control for molecular beacon based studies, we have quantified the changes in Oct-4 mRNA level 4 days after RA treatment in P19 cells.
To further establish the molecular beacon based method for detection and sorting of stem cells (including cancer stem cells and iPS cells), research is being conducted to demonstrate the sensitivity, specificity and safety of this technique.
In this study, specific primers and modified molecular beacon probes of nine specific virulence genes, whose 5′end were added with homo tail sequence, were designed; and a two-tube modified molecular beacon based multiplex real time PCR (rtPCR) assay for the identification of five Escherichia coli pathotypes, including ETEC, EAEC, EPEC, EHEC and EIEC was developed and optimized.
In a nutshell, the molecular beacon based PCR assay developed in the present study addresses to a large extent the need of a simple, cost effective, easy to use, highly sensitive, and specific nucleic acid amplification assay, a step forward towards developing point-of-care diagnostics for low resource settings [ 21, 22].
