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Several qrt-PCR assays, mostly based on SYBR Green I, Taqman and Molecular Beacon technologies, have been developed for a large number of toxic microalgal species quantification [1], [2], [3].
Commercial assays that use line-probe and molecular beacon technologies have been produced that are rapid and accurate (22, 23 ).
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Xpert uses molecular beacon technology to detect rifampicin resistance.
Xpert MTB/RIF assay is an automatic molecular test based on seminested real-time PCR and molecular beacon technology targeting the rpoB gene.
Primers used in PCR experiments with molecular beacons produced amplicons, which were between 70 and 82 base pairs (Table 1).
Here we explore the utility of molecular beacon real-time PCR technology for detection of virulent Bacillus anthracis strains.
Use of the molecular beacon real-time PCR technology should accelerate current efforts to swiftly detect B. anthracis strains and its virulence plasmids in clinical and environmental samples and may extend to the development of additional molecular beacon-based assays for the identification of other pathogenic agents or the identification of B. anthracis directly from clinical samples.
By combining the standard NASBA technology [ 28] with a molecular beacon that anneals during amplification to the target sequence, a real-time detection system is generated [ 29].
Compatible with most of oligonucleotide probes' designs and enzyme-based signal amplification strategies, the molecular beacon can serve as a novel signal translator to expand the application prospect of FA technology in various bioassays.
The probes were TaqMan, conventional molecular beacon (MB), and shared-stem molecular beacon (ATssMB and GCssMB).
Different parameters such as molecular beacon design, Au nano-particle size, molecular beacon nano-particle conjugation protocol, molecular beacon nano-particlell as experimental conjugationwere evaluated.
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