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Quantitative RT-PCR was performed using the molecular beacon technique by the Beacon Real-Time PCR Universal Reagent (Cat# GMRS-001, GenePharma, Shanghai) according to the manufacture's instructions.
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The merits of two techniques, molecular beacon and amplification effect of enzymatic color, are successfully combined, and the technique is simple, sensitive and specific, to detect and compare the methylenetetrahydrofolate reductase (MTHFR) Gene C677T mutation of subjects between coronary heart disease (CHD) and control group.
Several of the molecular techniques, Genotype MTBDR (Hain Lifescience) and molecular beacon assay, already test for the katG and inhA gene mutations that are associated with isoniazid resistance.
The probes were TaqMan, conventional molecular beacon (MB), and shared-stem molecular beacon (ATssMB and GCssMB).
Aptamer beacon is a promising technique to resolve this problem by combining the excellent protein binding specificity of the aptamer with the sensitive signal transduction mechanism of the molecular beacon.
Different parameters such as molecular beacon design, Au nano-particle size, molecular beacon nano-particle conjugation protocol, molecular beacon nano-particlell as experimental conjugationwere evaluated.
This paper reports an improved catalytic molecular beacon.
A molecular beacon (MB) was designed as a template.
A shared-stem beacon probe combines the properties of a TaqMan probe and a conventional molecular beacon.
The "molecular beacon" without fluorescence and quenching molecules is entitled as "hairpin DNA probe" by us for only the "hairpin" structure of traditional molecular beacon is adopted.
The produced triggers could be used to unfold molecular beacon (MB) and initiate T-EXPEA process.
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