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Microinjections were performed using an Eppendorf manipulator and Femtojet microinjector, delivering femtoliters of the molecular beacon solution.
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Without Hg2+, the report probes were stable as molecular beacons in solution.
In addition, performance of the beacon array biosensor was compared with molecular beacons in homogeneous solution.
The experiment results showed that this type of electrochemical active inactive switching aptamer molecular beacon allowed the direct detection of target proteins in the solution with no requirement of removing uncombined CAs-MB.
The molecular beacon was added to cells which had been fixed with a 4% paraformaldehyde solution, and the cells were incubated at 75°C for 10 min followed by incubation at room temperature for 1 h, then washed with PBS three times, with each wash lasting 10 min, and treated with 5% nonfat milk for 1 h.
We have also prepared molecular beacon DNA biosensor arrays for simultaneous analysis of multiple DNA sequences in the same solution.
Molecular beacon (MB) DNA probes provide a new way for sensitive label-free DNA/protein detection in homogeneous solution and biosensor development.
In this study, we developed a new class of amplification assay, double-hairpin molecular beacon (DHMB -based cascaDHMB -basedcascadeamplification (rollingor ultrasensitive and selecircledetection of Kramplificationhomogenous solution.
The probes were TaqMan, conventional molecular beacon (MB), and shared-stem molecular beacon (ATssMB and GCssMB).
Different parameters such as molecular beacon design, Au nano-particle size, molecular beacon nano-particle conjugation protocol, molecular beacon nano-particlell as experimental conjugationwere evaluated.
This paper reports an improved catalytic molecular beacon.
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