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One utilizes a molecular beacon consisting of two specific regions related to a DNAzyme and a substrate.
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Hence, in this study, we designed two molecular beacons consisting of a fluorescent dye peptide conjugate attached to gold nanoparticles (Au NPs) for in vitro and in vivo detection of the matriptase expression on tumor cells.
With the use of Human Immunodeficiency Virus type 1 (HIV-1) as a proof-of-principle analyte, we first demonstrated this approach by using a molecular beacon, which consists of a central section with the DNA sequence complementary to HIV-1, flanked by two arm segments.
The beacon consists of a coordinators timestamp and Block ACKnowledgment (B-ACK).
This beacon consisted of a biotinylated fluorophore-labeled DNA aptamer and a quencher-carrying complementary strand.
The beacon consisted of a plastic rod painted with alternating black and white hoops.
The beacon consisted of a fluorophore-labeled aptamer strand hybridized with a shorter, quencher-carrying complementary strand.
The molecular-beacon probe consists of three elements.
Molecular beacon (MB -based sensing platforMB -basedonsensing a fluorogen-quencher platforms an importhat role in mediconsist biofogical researches.
The two loops of this molecular beacon are designed in such a manner that they consist of thrombin (Tmb) aptamer sequence and adenosine triphosphate (ATP) aptamer sequence, respectively, which are utilized to sense thrombin and ATP.
The probes were TaqMan, conventional molecular beacon (MB), and shared-stem molecular beacon (ATssMB and GCssMB).
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