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While techniques exist for the identification of NoV, the lack of a reliable cell culture system, NoV genetic variability, and time-consuming sample preparation steps required to isolate the virus (or its genome) prior to molecular based methods has hindered rapid virus detection.
However, the differential increase appears less marked for molecular based methods [32].
For example, recent applications of molecular based methods have shown that individual pine seedlings can support several genotypes of Hebeloma cylindrosporum, and a typical m2 of forest floor contained ∼9 genotypes of this species [28], [29].
Molecular based methods are not themselves without bias, with such bias known in the amplification process which will impact on both PCR and RT-PCR steps [ 34, 35].
The reverse line blot hybridization assay (RLB) has been used to detect Mhf and CMhm in cats [ 18] however this assay has not been compared to other more sensitive molecular based methods.
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For suitable diagnosis and treatment, rapid and accurate identification of etiologic agents in clinical samples using reliable molecular based method is required.
The Johnson Lab has expertise in genomic, lipidomic, and molecular biology based methods for understanding how bioactive lipids shape host-microbe interactions.
Although molecular descriptor based methods are computationally simple and effective in practice but they share several shortcomings most important being the inability to identify local similarity between structures.
We used molecular clock based methods to estimate divergence times in our matK gene tree.
Even with an accurate topology, rate variation can bias the estimate of divergence times with molecular clock based methods.
Using MS/MS data, we corrected the translational start sites and UTR assignment of proteins, which would otherwise be difficult, or impossible using molecular biology based methods.
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molecular based technics
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molecular based methodologies
molecular based approaches
molecular based therapies
molecular based method
dna based methods
cell based methods
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