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Incorporating molecular barcodes, also known as unique molecular indices or UMIs, may prove useful for improving efficiency and distinguishing true mutations from PCR or sequencing errors [27].
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The sequence of the molecular barcodes can also affect variant calling performance.
Thus, it will be beneficial if molecular barcodes can also be applied in high multiplex PCR amplicon sequencing.
Finally, we have employed well selected 21 nucleotide molecular barcodes that are unambiguous in sequencing, which can also be counted by hybridization.
As the proteins were selected across the fungal kingdom and because they represent single KOG proteins, they may also be suitable for the development of molecular barcodes.
Good DNA codes are desirable for DNA computation, DNA microarray technologies and molecular barcodes, etc.
These sequences are specific to particular bird species, much like molecular barcodes.
Computerisation and the development of barcodes also made ticketing easier, faster and global.
Genomic DNA was isolated and molecular barcodes amplified by PCR.
Other approaches involve the use of exogenous molecular barcodes (or molecular tags) [ 8, 10, 11].
Molecular barcodes for all the Favia related sequences were downloaded from NCBI.
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