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Molecular assays based on the genetics of drug resistance may considerably reduce these turnaround times.
Molecular assays based on the polymerase chain reaction (PCR) have been proposed as an alternative for parasitic detection of T. cruzi infection in vectors [ 10].
Simple and inexpensive molecular assays based on dipstick and zipper technology have also been described.
It can furthermore be used to supplement other assays, including molecular assays based on other signature sequences, for definitive identification.
Until the molecular assays based on complete genome sequence of individual taxon become more readily available, identification and differentiation of the soft rot bacteria will continue to rely on the simultaneous use of several complementary probes/methods whenever possible.
To further understand the etiologic role of NoVs in sporadic diarrhea, we conducted a systematic review to identify studies that used similar inclusion criteria and molecular assays based on RT-PCR to detect NoVs in fecal specimens from patients with diarrhea.
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Molecular detection assays based on broadly reactive (degenerate) primers also tend to have differential and lower sensitivity for many flaviviruses.
In an assessment of molecular viral assays based on sensitivity, specificity and the density of the target virus in fecal-impacted samples, human adenovirus and human polyomavirus were found to be the most promising human-specific viral markers.
Molecular typing assays based on PCR amplification and sequencing of the AdV hexon and fibre genes were used.
Serial sections, immunohistochemical stains [ 45] and molecular marker assays (based on the reverse transcriptase-polymerase chain reaction technique) increase the detection rate of SLN metastases and could identifying micrometastases [ 46].
Objectives: In this study, a qualitative molecular assay based on automated RNA extraction with the MagNA Pure LC and real-time PCR on the LightCycler (LC) instrument was evaluated and compared with an in-house molecular assay.
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