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Objectives: In this study, a qualitative molecular assay based on automated RNA extraction with the MagNA Pure LC and real-time PCR on the LightCycler (LC) instrument was evaluated and compared with an in-house molecular assay.
The molecular assay based on RNA is quantitative to determine the level of ERα expression.
Gastric biopsy specimens of 68 peptic ulcer disease (PUD) and 327 chronic gastritis (CG) patients with a positive histological diagnosis of H. pylori were investigated for TetR 16 S rDNA genotype through a molecular assay based on amplification of a 16 S rDNA 545 bp fragment by polymerase chain reaction and H infI restriction fragment length polymorphism (PCR/RFLP).
Although this technique has proven to be useful, several drawbacks related to its sensitivity especially in the case of small instars and applicability to large numbers of insects and dead specimens have motivated researchers to look for a molecular assay based on the polymerase chain reaction (PCR) as an alternative for parasitic detection of T. cruzi infection in vectors.
The results of this semi-automated molecular assay based on the quantification of cytokeratin 19 (CK19) mRNA display a 96% concordance rate with detailed pathology complemented by immunohistochemistry when alternate slices of the same lymph node are used for the two tests (Cserni, 2012; Tamaki, 2012).
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Simple and inexpensive molecular assays based on dipstick and zipper technology have also been described.
Molecular assays based on the genetics of drug resistance may considerably reduce these turnaround times.
It can furthermore be used to supplement other assays, including molecular assays based on other signature sequences, for definitive identification.
Molecular assays based on the polymerase chain reaction (PCR) have been proposed as an alternative for parasitic detection of T. cruzi infection in vectors [ 10].
Until the molecular assays based on complete genome sequence of individual taxon become more readily available, identification and differentiation of the soft rot bacteria will continue to rely on the simultaneous use of several complementary probes/methods whenever possible.
To further understand the etiologic role of NoVs in sporadic diarrhea, we conducted a systematic review to identify studies that used similar inclusion criteria and molecular assays based on RT-PCR to detect NoVs in fecal specimens from patients with diarrhea.
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