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This led us to use a molecular approach using DN or OE PPARγ constructs to investigate whether the effects seen on cell proliferation and cell cycle were PPARγ dependent or independent.
Molecular methods performed on lymph node biopsy specimens are useful diagnostic tools, but the common single molecular approach using 16S rRNA PCR lacks sensitivity, which delayed diagnosis for this patient (3 ).
Highly active hydrodesulfurization (HDS) catalysts have been prepared successfully through a molecular approach using well-defined Strandberg PMoNi polyoxometalates (POMs) as superior precursors.
We observed that there was no statistically significant difference between the results from molecular approach using real time PCR and results obtained by the MG-RAST based taxonomic analysis of the shotgun metagenome sequence data, except for Prevotella.
In 2001, a novel molecular approach using microarrays to monitor genome-wide changes in relative mRNA abundance in the host response to infection was described [ 53].
Recently, Vigfussdottir et al. [ 10] tried to unravel the underlying processes among these two biphyletic gull species with a combined molecular approach (using mtDNA sequence haplotypes and autosomal microsatellite genotypes) from argentatus and hyperboreus breeding in Iceland and Greenland.
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Our staged molecular approach used complementary tools for pathogen detection and discovery that include syndrome-based multiplex PCRs, DNA microarray, and high-throughput sequencing (18 ).
Such patterns could vary according to the specific molecular approach used for constructing (Meyer et al., 2012) and/or amplifying (Ginolhac et al., 2011) second-generation DNA libraries.
Finally, the molecular approach used in this study, involving the combination of a transformed ITS dataset and DNA-pooling of a moderate number of infected host specimens, proved to be a simple but reliable method to address parasite population differentiation over space and time (see also [ 40]).
Recently, molecular approaches using cDNA microarrays and differential display have provided useful tools for further understanding of the molecular mechanisms that contribute to cisplatin resistance (Grottke et al, 2000; Niedner et al, 2001).
In the era of 'omics', molecular approaches using either specific or degenerate primers are still of high importance, especially in ecological studies where many samples need to be investigated or screened.
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