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PCR products with positive bands on a 1.5% agarose gel were purified using an Invisorb fragment cleanup kit by Stratec Molecular and subsequently directly sequenced at the Beijing Genomics Institute.
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However, these molecular signatures suggest that molecular typing and, subsequently, prognosis and treatment considerations for breast cancer patients may require incorporation of specific GATA3 mutations.
In 1987 Greider received a Ph.D. in molecular biology and subsequently was awarded a fellowship to conduct research at Cold Spring Harbor Laboratory in New York.
It occurs when a molecule whose normal ground state is a singlet is excited to a higher singlet state, goes to a vibrationally excited triplet state via either an intersystem crossing or a molecular collision, and subsequently, following vibrational relaxation, decays back to the singlet ground state by means of a forbidden transition.
They used 2,7-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) as a molecular probe and subsequently the fluorescence intensity was determined using a microplate reader under excitation/emission at 485/530 nm.
This will potentially guide treatment based solely on molecular measurements and subsequently eliminate the need for culture methods.
Cerebrospinal fluid samples were stored at −80°C for further viral and bacterial molecular investigations and subsequently tested in an Australian nationally accredited laboratory.
Residues of the CycT1 TRM were omitted from the model used for molecular replacement and subsequently built into the omit electron density.
We subjected microfibrils extracted from control and diabetic aortas to molecular combing and subsequently quantified their ability to resist an applied surface tension tensile force of ∼4000 pN by measuring microfibril periodicity in AFM height images [26].
Norephedrine (1), 2-amino-1,2-diphenylethanol (6), and 2-amino-1-indanol (9) were treated with pyridine-2-carbaldehyde in the presence of activated molecular sieves and subsequently reduced with sodium borohydride to obtain the chiral ionic liquid precursors.
Access to such information is essential in order to understand how genetic alterations affect gene products at the molecular level and subsequently to elucidate the relationships between genotypic and phenotypic variations.
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