Molecular analyses were taken as a gold standard.
Serum biochemical, bone histomorphometric, and molecular analyses were performed.
Molecular analyses were done in the Laboratory of Molecular Biology, Zoology Department, Faculty of Science, Al Azhar University, Cairo.
For this purpose, serological and molecular analyses were employed to detect PVX in inoculated N. benthamiana by ELISA, Western blot, and RT-PCR.
Molecular analyses were conducted with the sandwich hybridization assay (SHA), which employed 18S ribosomal RNA oligonucleotide probes designed to detect calanoid and podoplean copepods, and the larvae of barnacles, mussels, polychaete worms, brachyuran crabs, and invasive green crabs (Carcinus maenas).
All primary cell cultures used for the molecular analyses were from passages ≤6.
Rock samples intended for molecular analyses were maintained at −80°C until the time of analysis.
Molecular analyses were performed on tissue sample from primary tumor used for initial diagnosis in 130 out of 132 cases.
Additionally, identical specimens for molecular analyses were dried in frozen silica gel, placed in hermetically sealed vials, and stored in a freezer.
In order to examine variability in our DNA extraction process, split samples of oropharyngeal and sputa specimens were prepared and molecular analyses were run in parallel on the duplicate DNAs.
Serological and molecular analyses were performed.
