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Because both alleles displayed similar early flowering behaviors, atjmj4-1 waselecteded for the genetic and molecular analyses we report herein.
For the morphometric and molecular analyses, we collected samples of S. biwaensis and S. v. microoculus from the entire shoreline of Lake Biwa between 2002 and 2007 (Figure 1A; Table 1).
The total numbers of samples used in morphometric analyses were 508 and 370 from a total of 15 sites for head and body shape analyses, respectively, whereas for the molecular analyses, we used 207 and 181 samples from a total of 14 sites for the mtDNA and microsatellite analyses, respectively (see Table 1 for details).
Using a combination of flow cytometry, polarographic and molecular analyses, we evidenced a marked decrease in the cardiolipin content of R1H cells cultured in growth and differentiation media, together with a significant increase in the content of mitochondrial biogenesis factors and mitochondrial respiratory chain proteins.
For technical details on DNA extraction and molecular analyses we refer to a previous publication [ 32].
For more detailed molecular analyses, we sought to establish a cellular model.
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In order to conduct the molecular dating analyses we used the same 12-marker molecular dataset as [ 33].
Based on detailed morphological study, both of herbarium materials and living collection, and cytological and molecular phylogenetic analyses, we confirmed that the unknown Begonia is a new species, which is hereby described as Begonia jinyunensis C.-I Peng, B. Ding & Q.
In our molecular phylogenetic analyses, we used specific decisive datasets for each of our phylogenetic questions.
Given the inconsistent results among molecular phylogenetic analyses, we assessed whether rare genomic structural changes could provide further insight into fern relationships.
For the ortholog groups used for molecular evolution analyses, we then extracted the portion of the alignments with representation from all taxa.
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