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Mycelia subjected to this high-stringency selection procedure were used as starting material for further molecular analyses (see below).
(For further details on the molecular analyses, see Text S1).
This was later confirmed by further serological characterization and molecular analyses (see below).
Molecular analyses (see [ 41]) have so far identified 41 Caucasian cases carrying a deleterious germline mutation in either BRCA1 or BRCA2, and these subjects have been excluded from the analyses.
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If signs of beginning destabilization appear, monitoring should be intensified, and more specific parameters such as characteristic SCFA and molecular microbiology analyses (see 'Changing process conditions and microbial community structure' and 'Microbial bioindicators and early warning systems' sections) may be included.
A loon/grebe clade has been repeatedly tested and rejected by molecular phylogenetic analyses (see review by Mayr [140]), with grebes strongly supported as the sister taxon to flamingos, and this clade recovered well outside of a monophyletic waterbird assemblage.
This result is in conflict with a growing consensus on eukaryotic relationships from other molecular phylogenetic analyses (see [ 10] for review).
This means that divergence times obtained from other sources, i.e., [ 24, 36], possibly overestimate telmatherinid and melanotaeniid clade ages, again highlighting the issues related with molecular clock analyses (see Discussion above).
A total of 73 nucleotide and 90 peptide sequences were aligned using the default settings in Geneious Pro, version 3.8.5 [ 72] then manually adjusted for optimal alignment prior to the following molecular evolution analyses (see Additional file 1: Figure S1 and S2).
The appearance of these cells suggests that they are in the testis (a result confirmed by molecular analyses. See the later paragraph), hence we designated them as testis ova.
Such revisions will ideally integrate molecular, conchological and anatomical analyses (see below).
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