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The results revealed the efficiency of NGS derived IT marker development and indicated their utility in diverse molecular analyses including their applicability for cross-species studies.
However, Ruppert's hypothesis has not been substantiated by past molecular analyses, including that of Todaro et al. [11], which found the family in a sister group relationship with a cluster of chaetonotidans, although with low statistical support.
RM performed molecular analyses including PCR and DNA sequencing.
31 Therefore, more precise mechanisms should be elucidated by further molecular analyses including whole exome sequencing and comprehensive expression analyses.
The extensive biological material being collected will be subjected to further molecular analyses, including exploration for plasma-based mutations and detection, and molecular profiling of circulating tumour cells.
BH carried out the sample collection and processing, molecular analyses including fine mapping, cloning, and laboratory-based confirmation of microarray results, and drafted the manuscript.
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Furthermore, molecular analyses include species-related differences in probe permeability, and amplification properties, and because of the relatively small number of probes available for analyzing the many uncharacterized gut species [19], we cannot exclude the possibility that we may have missed other significant differences in microbial density.
Our molecular analyses included one individual of Chaetomys subspinosus from the state of Bahia in northeastern Brazil, and other hystricognaths.
The whole series of HCA used for the different molecular analyses included 35 H-HCA previously described [ 8, 9, 17], and 23 normal livers taken from patients resected with primary liver tumors developed in the absence of cirrhosis.
Molecular genetic analyses, including the McDonald-Kreitman test, were performed with DnaSP [ 53].
Plasma and liver samples were collected for the biochemical and molecular biological analyses, including both immunohistochemical and histological studies.
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