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The ability to extract adequate material for global molecular analyses from smaller clinical samples paves the way for less invasive sample collection techniques within the clinic, for example a 'finger pick' for blood versus a full phlebotomy procedure.
Written consent was obtained for studies of human specimens and ethics approval was obtained for further molecular analyses from the Human Research Ethics Committe of the Menzies School of Health Research and Department of Health and Families.
The concentrations of 0.5% sucrose, 0.5% sucrose +1% starch and 1% sucrose were selected based on biochemical and molecular analyses from our preliminary studies (see data in Appendix S1) and previously published data [15], [16], [21].
AV conducted the fine dissections of the female ovaries and molecular analyses from DNA purification, cloning, up to sequencing.
Samples for molecular analyses from batches B.6 and C.6 were taken after 6, 13, and 40 days of incubation.
Molecular analyses from blood and buccal smear did not detect any mtDNA mutation responsible for any of LHON, MELAS or MERRF syndromes.
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All primary cell cultures used for the molecular analyses were from passages ≤6.
For the molecular analyses, beetles from four control lines and their corresponding, paired coevolved lines were chosen.
For molecular analyses, samples from batch A.6 were taken before the addition of pure tNP (t = 15 days) and also after 17, 28, and 55 days.
On the other hand, it should be noted that molecular analyses derived from placebo-controlled studies (BR.21 and ISEL) were retrospective, not preplanned and restricted to patient subsets with available samples and thus likely to be biased (McShane et al, 2005).
Further evidence for zoonotic transmission comes from molecular analyses of DNA extracted from 11 Ascaris spp. worms recovered from patients in Cornwall.
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