Bet v 1.0401 batch A showed a clear tendency to form high molecular weight aggregates ranging from smaller oligomers (12%) to large multimeric complexes (2%), whereas more than 99% of batch B appeared monomeric when analyzed by online HPSEC-light scattering.
For the various solvent mixtures, the weight-averaged molecular weights, Mw of the PMMA-b-C60 aggregates range from 4.1×107 to 12.5×107 g/mol.
Aggregates range from extremely dense amyloidogenic aggregates with β-sheet cores (Htt, ataxin-3, Aβ) to more amorphous aggregates (α-synuclein, SOD1, TDP-43).
The scFvs displayed an ability to form higher molecular aggregates in vivo.
LiBr (40 mM) was added to the DMF mobile phase to dissociate molecular aggregates of polymer during GPC analysis.
However, The ICG in PFC/ICG nanomulsions maintains the chemical stabilization and reduces the ICG molecular aggregates.
The small molecules wrapped inside the heavy oil molecular aggregates are released and the viscosity undergoes reversible reduction.
The identification of brightness with molecular aggregates is based on the hypothesis that the interactions in the aggregates do not modify the molecular fluorescence.
Thus, the dialysed samples lack the smallest molecular weight fractions while the sonicated ones lack also molecular aggregates, if present.
However, at the resolution of our microscope it is not possible to see smaller molecular aggregates.
