Sentence examples for molecular Probes protocol from inspiring English sources

The probes were labeled by a chemical reaction described in the Molecular Probes protocol (Invitrogen).

Anti-Rb G3-2455, BD) antibody was conjugated to the Alexa Fluor by mAb labeling kits according to the supplier's (Molecular Probes) protocol.

Labeling efficiency was visualized by fluorescence microscopy and bacteria were additionally labeled with Syto 9 (Invitrogen, Darmstadt, Germany) for co-registration according to molecular probes standard protocols.

Amplex Ultra Red detects H2O2, which is formed from free glucose by glucose oxidase (Invitrogen, Molecular Probes Amplex UltraRed protocol) in the reaction solution.

Fractions were estimated to be at least 95% pure using SYPRO orange staining (molecular probes S5692, per protocol, sensitive to 5 ng of protein) with phosphoimager detection.

Mitochondria membrane potential was detected using MitoTracker® Red CMXRos according to manufacturer's protocol (Molecular Probes).

MP was labeled with Oregon green in sodium bicarbonate buffer (pH 8.0) following the manufacturer's protocol (Molecular Probes).

Plasma membrane labeling of live adherent cells and isolated fraction of microparticles was done using Lipophilic Styryl dye FM® 1-43FX (Molecular Probes, USA) according a protocol recommended by the manufacturer.

To assess mitochondrial superoxide production, NRCM were loaded with 0.5 µM MitoSox (Molecular Probes) using the same protocol.

After rinsing in TBST and TBS, DAPI staining was performed according to the manufacturer's protocol (Molecular Probes).

Incorporation of bromodeoxyuridine (BrdU) during DNA synthesis was evaluated with the Click-iT™ EdU flow cytometry assay kit, following the manufacturer's protocol (Molecular Probes, Eugene, OR, USA).