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Cholera toxin β subunit (CTxB) Alexa-555-conjugated was from Molecular probes (code: C-34776).
The detection of the biotinylated secondary antibody was by using streptavidin-Alexa 594 (1:1000 Molecular Probes code S-11227; Molecular Probes, Eugene, Oregon, USA).
For co-localisation of syndecan-3 and CD68, sections were incubated with anti-syndecan-3 (5 μg/ml) and anti-CD68 (4 μg/ml), washed in PBS and treated with goat anti-mouse IgG-Alexa 488 (1:500 Molecular Probes code A11001) and goat anti-rabbit-Texas red (1 100 Santa Cruz Biotechnology code sc-2780) containing 10% human serum.
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CellTrace™ CFSE stock solution (code C34554, Molecular Probes- Invitrogen, Eugene, OR, USA) was prepared in dimethyl sulfoxide according to manufacturer's instructions.
Molecular probes were designed to detect genes coding for various oxygenase systems.
Multidimensional optical coding has been developed to assign addressable molecular probes for multiplexed molecular and cellular sensing.
Invitrogen Block-iT RNAi Designer (Invitrogen, Molecular Probes, Carlsbad, CA) was used to design these 21 nucleotide sequences targeting human SOD1 coding sequence, and no other known sequence in the mouse genome.
AccuCheck Counting Beads (Molecular probes).
E. The orientation freedom of molecular probes.
Nuclei were stained with Hoechst 33258 (Molecular Probes).
DND Green-189 (L7535) was purchased from Molecular Probes.
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