Sentence examples for molecular Biochemicals containing from inspiring English sources

After centrifugation (300× g for 5 min), the cell pellet was digested in 1 mg/mL of collagenase-dispase (Roche Molecular Biochemicals) containing 1 mg/mL of DNase (Sigma) and 0.15 mg/ml of Na-p-tosyl-L-lysine chloromethyl ketone (Sigma) for 1 h at 37°C.

Retinal pieces were incubated in papain (0.2 mg/ml; Roche Molecular Biochemicals, Mannheim, Germany -containing Ca2+- and MGermany -containingbuffered saline, pH 7.4, for 30 minutes at 37°Ca2+ollowed by several washing steps with saline.

For the luciferase assays of the fos and EGR1 reporter plasmids, on the day of transfection, SkBr3 cell medium was replaced with RPMI without phenol red and serum, and transfection was performed using X-tremeGENE 9 DNA Transfection Reagent (Roche Molecular Biochemicals, Milan, Italy) and a mixture containing 0.5 μg of each reporter plasmid and 5 ng of pRL-TK.

Isolated retinas were washed with sterile ice-cold PBS containing CPI (Roche Molecular Biochemicals).

Cells were stained with 5 micrograms/ml propidium iodide (Sigma) containing RNase A (Roche Molecular Biochemicals, Mannheim, Germany).

In this system, all reactions were run in glass capillaries with the LightCycler TaqMan Master Mix (cod. 04735536001, Roche Molecular Biochemicals), in a volume of 20  μL containing 2  μL of cDNA and 1  μL of specific TaqMan Gene Expression Assays for human SPARC (RealTime Designer Assay cod. 04162498001, Roche Molecular Biochemicals), according to the manufacturer's directions.

Swabs were collected into sterile tubes containing 400 μl DNA/RNA stabilization buffer (Roche Molecular Biochemicals, Indianapolis, IN, USA) and stored at − 80 °C until the DNA was extracted.

The cells were suspended in 8 ml wash buffer (50 mM NaH2PO4, 0.3 M NaCl, pH 7.5) containing protease inhibitor cocktail EDTA-free (Roche Molecular Biochemicals, Mannheim Germany), and the suspension was disrupted by ultrasonication.

Brain tissue was homogenized in 10 vol of 50 mM Tris (pH 8,0), 150 mM NaCl and 5 mM EDTA containing a proteinase inhibitor cocktail (Complete, Roche Molecular Biochemicals, Indianapolis, IN, USA).

The homogenization buffer also contained cocktails of protease inhibitors (Protease Inhibitors Complete; Roche Molecular Biochemicals) and protein phosphatase inhibitors (Protein Phosphatase Inhibitor Cocktail I and II; Sigma Aldrich).

After incubation with reaction buffer containing terminal deoxyribonucleotidyl transferase according to the manufacturer's protocol (Roche Molecular Biochemicals), the slides were stained with ABC reagent.