Integration and analysis was performed using Molecular Analyst software program (Bio-Rad, Hercules, CA, USA).
Calculation of the similarity matrix and the creation of dendrograms was carried out with the Molecular Analyst® software program (Bio-Rad Laboratheies) unweightedunweighted pair group method and arithmetic averages.
The relative expression of the protein bands of IκB-α (∼37 kDa), Bax (∼23 kDa), Bcl-2 (∼29 kDa) was quantified by densitometric scanning of the X-ray films with GS-700 Imaging Densitometer (GS-700, Bio-Rad Laboratories, Milan, Italy) and a computer program (Molecular Analyst, IBM), and standardized for densitometric analysis to β-actin levels.
DNA was quantified with Gel Doc 1000 (Biorad) gel documentation system and the program Molecular Analyst (Biorad).
The relative expression of the protein bands of IκB-α (~37 kDa), iNOS (~130 kDa), NF-kB p65 (65 kDa), Bax (~23 kDa), Bcl-2 (~29 kDa) was quantified by densitometric scanning of the X-ray films with GS-700 Imaging Densitometer (GS-700, Bio-Rad Landratories, Milan, Italy) and a computer program (Molecular Analyst, IBM).
Dendrograms of genetic relatedness were created with Molecular Analyst/Multi-Analyst programs (Bio-Rad) by using the unweighted pair group method with arithmetic averages, and a position tolerance of 1.5%.
Donald Rumsfeld's military analyst program violated federal anti propaganda statutes.
Intensities of the respective bands were examined by densitometric analysis (Scion Image Analyst program).
Autoradiographs were quantified by scanning densitometry (Molecular Analyst version 4 software; Biorad, Hemel Hempstead, UK).
