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The lines depict the relationship for the ratio between measured and BLM calculated toxicity (measured LC50, EC50) as well as the ratio between the molar sum of metals (Fe, Al) and DOC in four lakes.
Compound and optical variables was also investigated and it was found that the molar sum of Al and Fe was a better predictor of bioassay toxicity for both test species than Fe was alone and CSF appeared to correlate strongly with bioassay toxicity (Fig. 2).
Total DAPs consisted of the molar sum of total DMs and total DEs.
Findings were weaker for urinary MEHHP, MEOHP, and the molar sum of the three DEHP metabolites.
Because DEHP is metabolized primarily into MEHP, MEHHP, MECPP, and MEOHP, we used two summary measurements: a) total molar sum of all four DEHP metabolites and b) molar sum of three oxidative DEHP metabolites (MECPP, MEHHP, and MEOHP).
We created a summary DEHP metabolite measure (∑DEHP) by calculating the molar sum of MEHP, MEHHP, MEOHP, and MECPP.
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The molar sums of total diethylphosphates, dimethylphosphates, and dialkylphosphates were also computed.
We calculated molar sums of the three DEHP metabolites to obtain a summary measure of DEHP using a previously reported technique (Wolff et al. 2008).
Calculated molar sums of "low-molecular-weight phthalates" or "high-molecular-weight phthalates" or the sum of all phthalates also were not significantly associated with any hormones or semen quality variables (results not shown).
We determined the molar sums of the dimethyl-containing and diethyl-containing metabolites; their geometric mean concentrations were 49.4 and 10.5 nmol/L, respectively, and their 95th percentiles were 583 and 108 nmol/L, respectively.
We calculated the molar sums of individual metabolites (in micromoles per liter) of DEHP because they occur from the same parent phthalate, and also of HMWP and LMWP, as they represent similar sources of exposure and they are moderately to highly correlated (ΣDEHPm, ΣHMWPm, and ΣLMWPm, hereafter) (Valvi et al. 2015).
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