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Arrhenius molar rate of creation/destruction of species ith in reaction r.
The latter (in a mixture of EtOH/H2O = 1 1.5 at 150 °C for 24 h) are from a non-stoichiometric synchronous feeding at a molar rate of MLi2O/MTiCl4 = 1.8 1.
The latter (in a mixture of EtOH/H2O = 1 1.5 at 150 °C for 24 h) are from a simple stoichiometric non-synchronous feeding where the equivalent mole of Li2O and TiCl4 solutions were fed at a molar rate of MLi2O/MTiCl4 = 2 1.
The solution was then transferred into a three-necked flask and deaerated by N2 bubbling for 30 min. Under stirring, 2.5 mM of oxygen-free NaHTe solution, which was freshly prepared from tellurium powder and NaBH4 (molar rate of 1: 2) in water at 0°C, was injected into the three-necked flask.
The solution was placed in a three-necked flask deaerated by N2 bubbling for 30 min. Under stirring, 2.5 mM of oxygen-free NaHTe solution, which was freshly prepared from tellurium powder and NaBH4 (molar rate of 1 2) in water at 0 °C, was injected into the three-necked flask.
Calculations of the molar rate of appearance (Ra) for the Rubisco reactions were performed according to Wolfe and Chinkes [38] using the equation Ra = k*Q, where Q represents the pool size of the respective metabolite.
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The ratio of the molar rates of appearance was ∼4.0 (glycolate/3PGA).
Under the LC condition, the molar rates of appearance were 4.80 * 10−6 µmol min−1 µg−1 Chl and 1.21 * 10−6 µmol min−1 µg−1 Chl for glycolate and 3PGA, respectively.
The molar rates of appearance were 4.27 * 10−7 µmol min−1 µg−1 Chl and 1.18 * 10−5 µmol min−1 µg−1 Chl for glycolate and 3PGA, respectively.
Taking the pool sizes of both metabolites into consideration, we calculated the molar rates of appearance under chase conditions, which may serve as estimates of the rates of oxygenation (glycolate) and carboxylation (3PGA).
In addition, this mutant enabled an estimation of the rates of oxygenation and carboxylation under HC and LC chase conditions by quantification of the molar rates of 13C appearance in the glycolate pool compared to the 3PGA pool.
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