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For α-lactalbumin, a consumption of 59.2 ± 6.1% by the reaction with molar excesses of PAO varying from 1.4 to 21 can be derived from the chromatograms.
The effect of different molar excesses of TCEP on dimerisation of J591-scFv.
One mechanism, which is widely supported, postulates an enzymatic role for SK.HPm in the conversion of molar excesses of plasminogen to plasmin.
The specificity of the protein binding was demonstrated because their formation was inhibited by competition with molar excesses of the unlabeled homologous oligonucleotides (Fig. 5C, lane 3).
Experiments were carried out with 100-fold molar excesses of unlabeled specific probe that competed with NF-κB binding and mutant probe that did not, demonstrating the sequence specificity of NF-κB bands.
We conclude from these data that more than 10-fold molar excesses of recombinant Ro60 and/or La proteins over the levels of endogenous hY RNAs do not inhibit chromosomal DNA replication in vitro.
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The ICs represent the conjugation with 40-fold molar excess of LC-SPDP biolinker.
Then, the conjugates with 40-fold molar excess of biolinker passed from Ultra-100K centrifilterfilter.
The reaction was quenched by adding a large molar excess of βME and the proteins were purified again on SEC.
A molar excess of hIL-2 blocked the protein synthesis inhibition dose-dependently.
For acceptor labeling, a 30-fold molar excess of AlexaFluor546 C5 maleimide (acceptor A) was used and incubated for 1 h at room temperature.
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