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Opportunely they can re-open in the presence of functionalizing transfer agent, owing that the later is introduced in molar excess with respect to the macrocycle, finally yielding linear dihydroxytelechelic oligomers.
For stable transfectants, the plasmid of interest was co-transfected at a 10 1 molar excess with pCoPuro.
To form the SMN Gemin2 complex, SMN26 51 was combined in molar excess with Gemin295 280 and the complex was purified on Superdex-75.
Allyl bromide (3× molar excess with respect to –OH groups) (Acros Organics) dissolved in 30 mL of THF subsequently was added.
The unsolubilized peptides were mixed in a fivefold molar excess with the protein solution and incubated for 2 hr on ice.
Briefly, the H2Bub1, H3K4me2, and H3K79me2 antibodies were preincubated with a 10-fold molar excess with specific or nonspecific peptides at 4° for 16 hr, and indirect immunofluorescent imaging was performed as described below.
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Biotin-bound Tr was obtained by incubation of Tr with biotin (Acros Organics), in 4-fold molar excess compared with the number of binding sites, at 4 °C overnight.
For Complex 1, the IC50 value for the DBA/2J radiolabelled probe competed with unlabelled DBA/2J DNA was 5.07±0.39 (fold molar excess) and with unlabelled 129B6 DNA was 1.04±0.06.
Interestingly, β-casein peptides consistently induced DegP12 cages even when mixed in large molar excess (20×; with respect to DegPS210A monomers) (Fig. 5A).
GKRP is present in liver cells in a 2 3-fold molar excess compared with GK, and its expression is relatively independent of food intake and hormonal status.
The principal function of haptoglobin, which is normally present at a greater than 400-fold molar excess compared with free hemoglobin [ 2], is to scavenge free hemoglobin [ 3] which has been liberated into the plasma by intravascular haemolysis.
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