The ICs represent the conjugation with 40-fold molar excess of LC-SPDP biolinker.
Then, the conjugates with 40-fold molar excess of biolinker passed from Ultra-100K centrifilterfilter.
The reaction was quenched by adding a large molar excess of βME and the proteins were purified again on SEC.
A molar excess of hIL-2 blocked the protein synthesis inhibition dose-dependently.
For stabilisation against depolymerisation and for fluorescence imaging, F-actin was labelled with a molar excess of tetramethylrhodamine-isothiocyanate-phalloidin (Sigma-Aldrich)50.
For acceptor labeling, a 30-fold molar excess of AlexaFluor546 C5 maleimide (acceptor A) was used and incubated for 1 h at room temperature.
For the VEGF-C inhibition assay, 0.25 μg/ml of pro-VEGF-C was mixed with 0.6 μg/ml (a 10-fold molar excess) of VEGF-C-CT.
As Fig. 1C shows, by 40-fold molar excess of crosslinker, we could reach to optimum IT with almost eliminated free MAb.
Briefly, a 3-fold molar excess of rhodamine or Alexa488 maleimide derivatives (Molecular Probes) was incubated with pure recombinant proteins at 4 °C overnight.
The biological activity of the 9300 dalton PKI is blocked completely by a 5 fold molar excess of antibody.
A four-fold molar excess of Fmoc-protected amino acids to resin was used for coupling.
